mouse anti α tubulin  (Proteintech)

Journal: iScience

Article Title: Domain-autonomous MKLP1 modules divergently regulate mitotic fidelity and polyploidy in a holocentric system

doi: 10.1016/j.isci.2026.117149

Figure Lengend Snippet:

Article Snippet: mouse anti-α-tubulin , Proteintech, Wuhan, China , Cat#66031-1-Ig;RRID:AB_11042766.

Techniques: Transfection, Cloning, Imaging, Bicinchoninic Acid Protein Assay, Western Blot, Plasmid Preparation, Recombinant, Software

Journal: Advanced Science

Article Title: Arrayed Magnetic Accelerator Synergistically Enhances Orthodontic Tooth Movement via Microtubule Assembly‐Mediated Periodontal Remodeling

doi: 10.1002/advs.77447

Figure Lengend Snippet: SMFs enhance orthodontic mechano‐transduction via activation in microtubule‐integrin assembly of PDLCs. (a) Heat map for cluster analysis of differentially expressed genes (DEGs). (b) Bubble plot of GO enrichment results of DEGs. (c) Bubble plot of KEGG enrichment results of DEGs. (d) Relative FPKM values of ITGB1, ITGB7, ITGB9, CCL7, TNF and IL6 from RNA‐seq data. Mean ± SD. n = 3. ** p < 0.01, *** p < 0.001. (e) 12h and 24h Relative gene expression of ITGB1, ITGB2, ITGB3, IFNG, MCSF and TNF from qRT‐PCR. Mean ± SD. n = 3. ** p < 0.01, *** p < 0.001, **** p < 0.0001. (f) Representative immunofluorescence images of α‐Tubulin (green), Integrin‐β1 (red) (merged yellow) and nuclei (blue) in mechanical force applied PDLCs without and with SMFs. Scale bar: 10 µm. (g) Pearson's coefficient and Manders’ overlap coefficient (M1) for the colocalization analysis between α‐Tubulin and Integrin‐β1. Mean ± SD. n = 30. **** p < 0.0001.

Article Snippet: PDLCs were respectively incubated with the primary antibody for Vinculin (Proteintech, 26520‐1‐Ap, 1:300), FAK (Proteintech, 12636‐1‐AP, 1:400), YAP/TAZ (Proteintech, 13584‐1‐Ap, 1:200), α‐Tubulin (Abclonal, AC012, 1:200) and Integrin‐β1 (Abclonal, A23497, 1:200).

Techniques: Transduction, Activation Assay, Analysis, RNA Sequencing, Gene Expression, Quantitative RT-PCR, Immunofluorescence

Journal: microPublication Biology

Article Title: A commercially available DJ-1 antibody does not detect C. elegans DJR-1.1 or DJR-1.2 proteins in whole worm lysates

doi: 10.17912/micropub.biology.002375

Figure Lengend Snippet: Top panel) Western blot showing non-specific bands detected by the anti-DJ-1 antibody NB300-270 in C. elegans. Whole worm lysates from djr-1.2 ( tm1346 ) , djr-1.2 ( tm951 ), and djr-1.1 ( tm918 ) exhibit bands identical to those observed in wild-type ( N2 ) whole worm lysates. Bottom panel) Housekeeping protein α-tubulin demonstrates equal loading of samples. The image shown is representative of three independent experiments.

Article Snippet: The membranes were blocked using a blocking buffer (1× Tris-buffered saline, 0.5% Fish Gelatin, 0.1% Tween 20, 0.02% Sodium Azide) then incubated with rabbit anti-DJ-1 antibody (1:1000; Novus Biologicals, NB300-270) overnight at 4°C or a mouse anti-alpha tubulin antibody (1:200; Santa Cruz Biotechnology, SC-32293) at room temperature for several hours.

Techniques: Western Blot

Journal: microPublication Biology

Article Title: A commercially available DJ-1 antibody does not detect C. elegans DJR-1.1 or DJR-1.2 proteins in whole worm lysates

doi: 10.17912/micropub.biology.002375

Figure Lengend Snippet:

Article Snippet: The membranes were blocked using a blocking buffer (1× Tris-buffered saline, 0.5% Fish Gelatin, 0.1% Tween 20, 0.02% Sodium Azide) then incubated with rabbit anti-DJ-1 antibody (1:1000; Novus Biologicals, NB300-270) overnight at 4°C or a mouse anti-alpha tubulin antibody (1:200; Santa Cruz Biotechnology, SC-32293) at room temperature for several hours.

Techniques: